2 edition of Characteristics of two salmonid fish cell lines found in the catalog.
Characteristics of two salmonid fish cell lines
Karl Terrence Kleeman
Written in English
|Other titles||Salmonid fish cell lines.|
|Statement||by Karl Terrence Kleeman.|
|The Physical Object|
|Pagination||, 122 leaves, bound :|
|Number of Pages||122|
Salmonid alphavirus (SAV) causes pancreas disease and sleeping disease in Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) and confers a major burden to the aquaculture industry. A commercial inactivated whole virus vaccine propagated in a salmon cell line at low temperature provides effective protection against SAV by: VHSV-IVb were studied in two rainbow trout cell lines, RTgill-W1 from the gill epithelium, and different physical characteristics. The effect of Corexit , a dispersant used to clean up oil spills, on invertebrates, lower vertebrates, birds and human health have been examined but there Fish cell lines and the study of fish.
characteristics and functions of shbgb, a newly discovered sex hormone binding globulin expressed in granulosa cells marivin, e. et al. zebra fish liver zfl cell line, an in-vitro model for the assessment of tbt-induced steatosis in fish porte, c. et al. capacity for epa and dha synthesis in atlantic salmon at different life stages Østbye, t-k. Cultural characteristics of salmonid alphaviruses (SAV) - influence of cell line and temperature. Journal of Fish Diseases, (In press). McLoughlin, M.F. and Graham, D.A. ().
Salmon / ˈ s æ m ə n / is the common name for several species of ray-finned fish in the family fish in the same family include trout, char, grayling and are native to tributaries of the North Atlantic (genus Salmo) and Pacific Ocean (genus Oncorhynchus).Many species of salmon have been introduced into non-native environments such as the Great Lakes of North Class: Actinopterygii. Books dealing with fish and sea mammals -- either topically or with the word "fish" or the name of a species in their title. Score A book’s total score is based on multiple factors, including the number of people who have voted for it and how highly those voters ranked the book.
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Characteristics of two salmonid fish cell lines: glycolytic rates, CO₂ requirements and susceptibility to Reovirus and Reovirus-like agents Public Deposited. You do Author: Karl Terrence Kleeman. Characteristics of two salmonid fish cell lines: glycolytic rates, CO₂ requirements and susceptibility to Reovirus and Reovirus-like agents.
Nine permanent cell lines have been established from five species of salmonids native to America's Pacific Northwest. With the exception of a hepatoma from an adult trout, the lines were derived from normal tissues of embryonic or juvenile fish. Cells were routinely grown in Eagle's minimum essential medium with 10% fetal bovine by: Chapter 10 -Tissue Culture of Fish Cell Lines.
Two cell lines, primary and complimentary outlined in Table 2, will be used for each sample set. Additional cell lines may be used at the discretion of the testing laboratory (Table 2). All viral testing of salmonids and herring will be on both chinook salmon embryo (CHSE) and epithelioma File Size: KB.
Isolation of a retrovirus from two fish cell lines developed from chinook salmon (Oncorhynchus tshawytscha) with plasmacytoid leukaemia.
Teleost fish cell lines have been developed from a broad range of tissues such as ovary, fin, swim bladder, heart, spleen, liver, eye muscle, vertebrae, brain, skin.
Agersborg, H.P.K. () Salient problems in the artificial rearing of salmonid fishes, with special reference to intestinal fungisitosis and the cause of white‐spot disease. Trans Am. Fish. fabienne roux master thesis in ecotoxicology 1 i. content i.
content 1 ii. abstract 2 iii. list of abbreviations 3 1 introduction 4 testing and alternative methods 4 primary cultures vs. cell lines 5 fish cell lines 6 biomarkers 7 challenges and possible solutions 10 thesis aims 12 2 materials and methods 13 chemicals and consumables 13File Size: 1MB.
the present study three established salmonid cell lines, CHSE, Chum salmon heart -1 (CHH-1) and salmon head kidney -1 (SHK-1) cells, were compared for their ability to isolate SAV-1 from experimentally infected Atlantic salmon based on their growth characteristics and CPE development.
MATERIALS AND METHODS Cell cultures. Salmon, Trout and Char (Salmonidae) The Salmonidae family is native to the Northern Hemisphere, but several species have been introduced to New Zealand.
Some of these species, particularly brown and rainbow trout, have established very successfully here and support New Zealand’s reputation as an angling Eldorado. Recommended Cell Lines for Various Fish Species Refer to Table 1 in Chapter Virology, for a listing of recommended cell lines and incubation temperatures to detect target viruses.
III. Maintenance of Stock Cell Lines & Passage of Cell Monolayers A. MATERIALS Fish cell line of choice L-glutamine - File Size: KB. Fish cell lines are used for diverse research applications.
Torofugu rubripes (pufferfish) possess a small genome with little junk DNA, while Danio rerio (Zebrafish) have a nearly transparent body during early development and have been used to study toxicology, developmental biology, and specific gene function and signaling pathways.
ATCC offers fish cell lines from a variety of tissue sources. In this study the TO cell line, originating from Atlantic salmon head kidney leucocytes, is described with respect to enzyme cytochemistry, functional studies, reactivity with leucocyte specific antibodies and immune gene expression.
Pronounced characteristics of the TO cell line are the rapid adherence to the plastic growth surface, Cited by: Cultured fish cells currently have application in many fields of biological research, and teleost cell lines have been initiated from a broad range of tissues and organs.
Lines reported in the literature represent 74 species or hybrids from 34 families of fishes. A listing of these fish cell lines Cited by: The Atlantic salmon and trout of the genus Salmo give the family and order their names. Salmonids have a relatively primitive appearance among the teleost fish, with the pelvic fins being placed far back, and an adipose fin towards the rear of the back.
They are Class: Actinopterygii. Laboratory studies were carried out to investigate the cultural characteristics of salmonid alphaviruses (SAV) from Atlantic salmon (AS, Salmo salar) and rainbow trout (RT, Oncorhynchus mykiss), particularly in relation to cell line and temperature.
In an initial study, SAV was isolated from 12 viraemic sera and passaged in Chinook salmon embryo (CHSE‐) cells at 15 ° by: “Characterization of cell line is the first indispensible step after each cell line is generated for determining its functionality, authenticity, contamination,origin etc” “Morphology, Chromosome and DNA analysis have now became the major standard procedure for cell line identification” This cell line was used to measure viraemia of Salmonid alphavirus (SAV), the cause of Salmon Pancreas Disease (SPD), a serious disease in farmed Atlantic salmon.
Two SAV genotype 1 (SAV1) isolates were used in this study, F (tissue-culture adapted, from Ireland) and (from a field case in Scotland).Cited by: 8. If success is limited, double, triple or quadruple transgenic fish cell lines will be attempted sequentially. Four eukaryotic selection markers have been used successfully in fish cells and dual-expression vectors have been engineered giving a potential of eight transgenes being over-expressed in a quadruple transgenic cell line.
Nine permanent cell lines have been established from five species of salmonids native to America's Pacific Northwest. With the exception of a hepatoma from an adult trout, the lines were derived from normal tissues of embryonic or juvenile fish.
Cells were routinely grown in Eagle's minimum essential medium with 10% fetal bovine by:. Characteristics of Two Salmonid Fish Cell Lines: Glycolytic Rates, CO2 Requirements and Susceptibility to Reovirus and Reovirus-like Agents by Karl Terrence Kleeman A THESIS submitted to Oregon State University in partial fulfillment of the requirements for the degree of Doctor of Philosophy June Cellular agriculture is defined as the production of agricultural products from cell cultures rather than from whole plants or animals.
With growing interest in cellular agriculture as a means to address public health, environmental, and animal welfare challenges of animal agriculture, the concept of producing seafood from fish cell- and tissue-cultures is emerging as an approach to address Cited by: 1.Dr.
Robert Behnke is the man when it comes to salmonid knowledge. Pick up his book if you want to learn more about this particular family, “Trout and Salmon of North America”. Another great resource is Gary Marston’s Native Trout Fly Fishing blog, he has a Trout and Salmon species page with pictures and information of all those that he.